Dr Virginie Rozot
Affiliations
- Associate Member, Institute of Infectious Disease and Molecular Medicine
- Senior Research Officer, South African Tuberculosis Vaccine Initiative (SATVI), Division of Immunology, Department of Pathology
- Principal Investigator and member of the Steering Committee, Wellcome Centre for Infectious Disease Research in Africa (CIDRI-Africa).
Key Expertise
TB
Main Research Focus
Virginie Rozot is an infectious disease clinical immunologist with major research interests directed towards understanding the immuno-pathology of tuberculosis to identify immune correlates of risk and protection against TB using high dimensional tools. She developed and is supervising the Mass cytometry platform of the Institute.
Virginie is particularly interested at understanding the immuno-pathology of TB in human tissues and through a grant awarded by the Wellcome Leap, is leading a project aiming at understanding the role of lymph nodes in the containment or spread of TB disease using an arsenal of state-of-the-art immunology, microscopy and microbiology approaches in post-mortem tissues. Ultimately, the results of this study will have direct relevance to TB vaccine design and vaccination strategies.
Most Significant Paper Authored in 2024
Kiravu, A., Rozot, V., Cruywagen, L., Gutschmidt, A., DuPlessis, N., Nemes, E., & the POI-BAL study group. (2024).
The protocol by outlines a method for isolating, phenotyping, and quantifying mycobacteria-specific myeloid cells from human bronchoalveolar lavage (BAL) fluid using mass cytometry. This approach enables high-dimensional profiling of innate immune cells at the site of lung infection, which is critical for tuberculosis research. It details steps for BAL sample processing, cell stimulation with mycobacterial antigens, staining for CyTOF (mass cytometry), and data analysis.